通过约4.5小时的固相ELISA方法，对培养细胞上清、血清和血浆中的猪乙酰胆碱（ACh）水平进行定量检测。其包含大肠杆菌表达的重组猪乙酰胆碱（ACh）蛋白和抗重组猪乙酰胆碱（ACh）蛋白抗体。该免疫检测显示能精确定量重组因子，用自然猪乙酰胆碱（ACh）检测的结果显示，计量反应曲线与试剂盒内重组蛋白标准品所做曲线相一致，说明该试剂盒能用于决定自然猪乙酰胆碱（ACh）的相对量。

The quantikine swine acetylcholine（ACh） immunoassay is a 4.5 hour solid phase elisa designed to measure acetylcholine（ACh） levels in cell culture supernates, serum, and plasma. It contains e. Coli-expressed recombinant swine acetylcholine（ACh） and antibodies raised against recombinant swine acetylcholine（ACh）. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural swine acetylcholine（ACh） showed dose response curves that were parallel to the standard curves obtained using the recombinant quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural swine acetylcholine（ACh）