- 血清--国产血清
- 血清--PAA胎牛血清
- 血清--GIBCO胎牛血清
- 血清--Hyclone胎牛血清
- 培养基--Gibco培养基
- 培养基--Hyclone液体培养基
- 生化试剂--进口试剂
- 生化试剂--其他
- 实验耗材--大龙移液器
- 实验耗材--雷勃移液器
- 实验耗材--百得移液器
- 实验耗材--Axygen耗材
- 实验耗材--康宁耗材
- 实验耗材--Eppendorf移液器
- 实验耗材--其他
- ELISA试剂盒--兔ELISA试剂盒
- ELISA试剂盒--猪ELISA试剂盒
- ELISA试剂盒--羊ELISA试剂盒
- ELISA试剂盒--鸡ELISA试剂盒
- ELISA试剂盒--牛ELISA试剂盒
- ELISA试剂盒--犬ELISA试剂盒
- ELISA试剂盒--鸭ELISA试剂盒
- ELISA试剂盒--猴ELISA试剂盒
- ELISA试剂盒--人ELISA试剂盒
- ELISA试剂盒--小鼠ELISA试剂盒
- ELISA试剂盒--大鼠ELISA试剂盒
- ELISA试剂盒--豚鼠ELISA试剂盒
- ELISA试剂盒-其他
- Omega试剂盒
- 德国Qiagen试剂盒
- 细胞生物试剂--Peprotech细胞因子
- 植物ELISA试剂盒
- 鱼ELISA试剂盒
- 试剂盒
- ELISA检测试剂盒
- 美国SANCHEZ ELISA 试剂盒
- SeraPro胎牛血清
- 生物试剂
- Axygen试剂盒
- Amresco试剂
- 对照品
- 凯杰产品
- 生化测试盒
- 无血清培养基
- 抗体
- 细胞
- 干粉
- DNA
- 天根试剂盒
- 天根试剂
- BD耗材
- 酶联免疫试剂盒
- 定量分析试剂盒
- Thermo移液器
- 百得单道移液器
- 百得移液器
- life试剂
- Nunc耗材
- 北京杰辉博高生物技术有限公司
- 联系人:杨经理
- 电话:4000610918
- 邮箱:1261299569@qq.com
- 地址:北京市海淀区乾通天商务楼
- 品牌:life
- 发布日期: 2019-10-09
- 更新日期: 2023-04-25
品牌 | life |
货号 | 18064014 |
纯度 | % |
规格 | 10,000 units |
保质期 | 6个月 |
是否进口 | 是 |
货号: 18064014
规格
Optimal Reaction Temperature: | 42° C |
---|---|
Final Product Size(s): | 12.3 kb or less |
Reverse Transcriptase: | SuperScript® II |
Ribonuclease H Activity: | Reduced |
Form: | Frozen |
Format: | Tube(s) |
Template: | ssDNA, ssRNA, RNA-DNA Hybrid |
Sensitivity: | Medium |
Product Size: | 10,000 units |
Concentration: | 200 U?μl |
Enzyme Function: | RNA dependent DNA Polymerase |
Shipping Condition: | Wet Ice |
Number of Reactions: | 50 Reactions |
内容及储存
SuperScript® II Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.
Store at -20°C. Guaranteed stable for 6 months when properly stored.
描述
SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics.
? Yield – Reduced RNase H activity results in greater first-strand cDNA yields
? Size of cDNA – Generation of RT-PCR products up to 12 kb
? Thermostability – Full activity at 42°C
? Flexibility – Uses either total or poly(A)+ RNA
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of SuperScript® II is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as template?primer (3).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.