产品分类
联系方式
  • 北京杰辉博高生物技术有限公司
  • 联系人:杨经理
  • 电话:4000610918
  • 邮箱:1261299569@qq.com
  • 地址:北京市海淀区乾通天商务楼
Jackson/Cy™3 AffiniPure Bovine Anti-Goat IgG (H+L)/0.5 mg/80
  • 品牌:Jackson
  • 发布日期: 2019-11-29
  • 更新日期: 2023-04-25
产品详请
品牌 Jackson
货号 805-165-180
纯度 %
规格 0.5 mg
保质期 一年
是否进口
  • 商品编号:805-165-180

  • 品牌:Jackson

  • 公司:Jackson ImmunoResearch Laboratories

  • 公司分类:Whole IgG Affinity-Purified Antibodies

  • Target:Goat

  • Host:Bovine

  • Specificity:IgG (H+L)

  • Conjugate:Cyanine Cy™3

  • Unit:0.5 mg

  • Antibody_Format:Whole IgG

  • Clonality:Polyclonal

  • Minimal_Cross_Reactivity:Bovine, Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat

WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonimmunoelectrophoresisand/orELISA ,theantibodyreactswithwholemoleculegoatIgG.Italsoreactswiththelightchainsofothergoatimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.TheantibodyhasbeentestedbyELISAand/orsolid-phaseadsorbedtoensureminimalcross-reactionwithbovine,chicken,guineapig,syrianhamster,horse,human,mouse,rabbitandratserumproteins,butitmaycross-reactwithimmunoglobulinsfromotherspecies.

PhysicalState:Freeze-driedsolid
Storage:

Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.


Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6
StABI lizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide

SuggestedWorkingConcentrationorDilutionRange:
Histo-/Cyto-Chemistry:-1:100-1:800
FlowCytometry:-1:100-1:800

Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.

CyanineCy™3

Amax:550Emax:570nm

Cy3isbrighter,morephotostable,andgiveslessbackgroundthanotherorange-redfluorescingdyeconjugates.Cy3conjugatescanbeexcitedmaximallyat550nm,withpeakemissionat570nm.Forfluorescencemicroscopy,Cy3canbevisualizedwithtrADI tionaltetramethylrhodamine(TRITC)filtersets,sincetheexcitationandemissionspectraarenearlyidenticaltothoseofTRITC.WerecommendCy3asabrighteralternativetoTRITC.Cy3canbeexcitedtoabout50%ofmaximumwithanargonlaser(514nmor528nmlines),ortoabout75%ofmaximumwithahelium/neonlaser(543nmline)ormercurylamp(546nmline).Cy3hasbeenusedwithfluoresceinfordoublelabeling;however,theuseofanarrowband-passemissionfilterforfluoresceinisrecommendedtominimizeCy3fluorescenceintheFITCfilterset.Cy3canalsobepairedwithAlexaFluor®647formultiplelabelingwhenusingaconfocalmicroscope.However,abetterchoiceformultiplelabelingisRhodamineRed-Xbecauseitsfluorescenceismidwaybetweenagreenfluorescingdye(likeAlexaFluor®488)andafar-red-fluorescingdyelikeAlexaFluor®647.