- 血清--国产血清
- 血清--PAA胎牛血清
- 血清--GIBCO胎牛血清
- 血清--Hyclone胎牛血清
- 培养基--Gibco培养基
- 培养基--Hyclone液体培养基
- 生化试剂--进口试剂
- 生化试剂--其他
- 实验耗材--大龙移液器
- 实验耗材--雷勃移液器
- 实验耗材--百得移液器
- 实验耗材--Axygen耗材
- 实验耗材--康宁耗材
- 实验耗材--Eppendorf移液器
- 实验耗材--其他
- ELISA试剂盒--兔ELISA试剂盒
- ELISA试剂盒--猪ELISA试剂盒
- ELISA试剂盒--羊ELISA试剂盒
- ELISA试剂盒--鸡ELISA试剂盒
- ELISA试剂盒--牛ELISA试剂盒
- ELISA试剂盒--犬ELISA试剂盒
- ELISA试剂盒--鸭ELISA试剂盒
- ELISA试剂盒--猴ELISA试剂盒
- ELISA试剂盒--人ELISA试剂盒
- ELISA试剂盒--小鼠ELISA试剂盒
- ELISA试剂盒--大鼠ELISA试剂盒
- ELISA试剂盒--豚鼠ELISA试剂盒
- ELISA试剂盒-其他
- Omega试剂盒
- 德国Qiagen试剂盒
- 细胞生物试剂--Peprotech细胞因子
- 植物ELISA试剂盒
- 鱼ELISA试剂盒
- 试剂盒
- ELISA检测试剂盒
- 美国SANCHEZ ELISA 试剂盒
- SeraPro胎牛血清
- 生物试剂
- Axygen试剂盒
- Amresco试剂
- 对照品
- 凯杰产品
- 生化测试盒
- 无血清培养基
- 抗体
- 细胞
- 干粉
- DNA
- 天根试剂盒
- 天根试剂
- BD耗材
- 酶联免疫试剂盒
- 定量分析试剂盒
- Thermo移液器
- 百得单道移液器
- 百得移液器
- life试剂
- Nunc耗材
- 北京杰辉博高生物技术有限公司
- 联系人:杨经理
- 电话:4000610918
- 邮箱:1261299569@qq.com
- 地址:北京市海淀区乾通天商务楼
- 品牌:Jackson
- 发布日期: 2019-11-29
- 更新日期: 2023-04-25
品牌 | Jackson |
货号 | 805-165-180 |
纯度 | % |
规格 | 0.5 mg |
保质期 | 一年 |
是否进口 | 是 |
商品编号:805-165-180
品牌:Jackson
公司:Jackson ImmunoResearch Laboratories
公司分类:Whole IgG Affinity-Purified Antibodies
Target:Goat
Host:Bovine
Specificity:IgG (H+L)
Conjugate:Cyanine Cy™3
Unit:0.5 mg
Antibody_Format:Whole IgG
Clonality:Polyclonal
Minimal_Cross_Reactivity:Bovine, Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat
WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.
Basedonimmunoelectrophoresisand/orELISA ,theantibodyreactswithwholemoleculegoatIgG.Italsoreactswiththelightchainsofothergoatimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.TheantibodyhasbeentestedbyELISAand/orsolid-phaseadsorbedtoensureminimalcross-reactionwithbovine,chicken,guineapig,syrianhamster,horse,human,mouse,rabbitandratserumproteins,butitmaycross-reactwithimmunoglobulinsfromotherspecies.
PhysicalState:Freeze-driedsolid
Storage:
Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.
Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6
StABI lizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide
SuggestedWorkingConcentrationorDilutionRange:
Histo-/Cyto-Chemistry:-1:100-1:800
FlowCytometry:-1:100-1:800
Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.
CyanineCy™3
Amax:550Emax:570nm
Cy3isbrighter,morephotostable,andgiveslessbackgroundthanotherorange-redfluorescingdyeconjugates.Cy3conjugatescanbeexcitedmaximallyat550nm,withpeakemissionat570nm.Forfluorescencemicroscopy,Cy3canbevisualizedwithtrADI tionaltetramethylrhodamine(TRITC)filtersets,sincetheexcitationandemissionspectraarenearlyidenticaltothoseofTRITC.WerecommendCy3asabrighteralternativetoTRITC.Cy3canbeexcitedtoabout50%ofmaximumwithanargonlaser(514nmor528nmlines),ortoabout75%ofmaximumwithahelium/neonlaser(543nmline)ormercurylamp(546nmline).Cy3hasbeenusedwithfluoresceinfordoublelabeling;however,theuseofanarrowband-passemissionfilterforfluoresceinisrecommendedtominimizeCy3fluorescenceintheFITCfilterset.Cy3canalsobepairedwithAlexaFluor®647formultiplelabelingwhenusingaconfocalmicroscope.However,abetterchoiceformultiplelabelingisRhodamineRed-Xbecauseitsfluorescenceismidwaybetweenagreenfluorescingdye(likeAlexaFluor®488)andafar-red-fluorescingdyelikeAlexaFluor®647.