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- 品牌：国产
- 货号：75707
- 价格： ￥10/瓶
- 发布日期： 2020-12-11
- 更新日期： 2023-04-25
| 品牌 | 国产 |
| 货号 | 75707 |
| 用途范围 | 科研实验 |
| 英文名称 | Cfr13I (Sau96I) (10 U/μL) |
| 纯度 | % |
| 保质期 | 一年 |
| 是否进口 | 是 |
Enzyme Cfr13I (Sau96I) 保存温度 -20℃ 货期 2-3天 Compatible Buffer 10x Buffer Tango Optimal Reaction Temperature 37° C Sensitive to Heat Inactivation: Yes Methylation Sensitivity CpG methylation-sensitive, Not dam methylation-sensitive, dcm methylation-sensitive 规格 1000units
Thermo Scientific Cfr13I (Sau96I) restriction enzyme recognizes G^GNCC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AspS9I, BmgT120I, PspPI, Sau96I). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
? Superior quality—stringent quality control and industry leading manufacturing process
? Convenient color-coded Five Buffer System
? Includes universal Tango buffer for double-digestions
? BSA premixed in reaction buffers
? Wide selection of restriction endonuclease specificities
Applications
? Molecular cloning
? Restriction site mapping
? Genotyping
? Southern blotting
? Restriction fragment length polymorphism (RFLP)
? SNP
Note: Cfr13I is blocked by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain, such as GM2163.
For methylation sensitivity, refer to product specifications.
