The original QuikChange Site-Directed Mutagenesis Kits eliminate the need for subcloning into M13-based bacteriophage vectors and for ss-DNA rescue. This makes site-directed mutagenesis studies simple and reliable since it allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. The XL version of the kit is specialized for efficient mutagenesis of large (4 -14 kb) or otherwise difficult-to mutagenize plasmid templates and features components specifically designed for more efficient DNA replication and bacterial transformation. The QuikChange Multi system allows mutagenesis at multiple sites (up to 5) in a single round, using a single oligonucleotide per site.

• >80% mutagenesis efficiency for 1 site; >55% mutagenesis efficiency for 3 mutations simultaneously

• Efficient and accurate linear amplification minimizes unwanted errors

• Free access to Primer Design Program

• QuikChange Lightning Live Webinar: Accelerate your research without sacrificing mutation efficiency, reliability and accuracy. Click here to view

New! Multiply the power of QuikChange to easily do scanning and combinatorial mutagenesis with QuikChange HT

For Research Use Only. Not for Use in Diagnostic Procedures.