- 血清--国产血清
- 血清--PAA胎牛血清
- 血清--GIBCO胎牛血清
- 血清--Hyclone胎牛血清
- 培养基--Gibco培养基
- 培养基--Hyclone液体培养基
- 生化试剂--进口试剂
- 生化试剂--其他
- 实验耗材--大龙移液器
- 实验耗材--雷勃移液器
- 实验耗材--百得移液器
- 实验耗材--Axygen耗材
- 实验耗材--康宁耗材
- 实验耗材--Eppendorf移液器
- 实验耗材--其他
- ELISA试剂盒--兔ELISA试剂盒
- ELISA试剂盒--猪ELISA试剂盒
- ELISA试剂盒--羊ELISA试剂盒
- ELISA试剂盒--鸡ELISA试剂盒
- ELISA试剂盒--牛ELISA试剂盒
- ELISA试剂盒--犬ELISA试剂盒
- ELISA试剂盒--鸭ELISA试剂盒
- ELISA试剂盒--猴ELISA试剂盒
- ELISA试剂盒--人ELISA试剂盒
- ELISA试剂盒--小鼠ELISA试剂盒
- ELISA试剂盒--大鼠ELISA试剂盒
- ELISA试剂盒--豚鼠ELISA试剂盒
- ELISA试剂盒-其他
- Omega试剂盒
- 德国Qiagen试剂盒
- 细胞生物试剂--Peprotech细胞因子
- 植物ELISA试剂盒
- 鱼ELISA试剂盒
- 试剂盒
- ELISA检测试剂盒
- 美国SANCHEZ ELISA 试剂盒
- SeraPro胎牛血清
- 生物试剂
- Axygen试剂盒
- Amresco试剂
- 对照品
- 凯杰产品
- 生化测试盒
- 无血清培养基
- 抗体
- 细胞
- 干粉
- DNA
- 天根试剂盒
- 天根试剂
- BD耗材
- 酶联免疫试剂盒
- 定量分析试剂盒
- Thermo移液器
- 百得单道移液器
- 百得移液器
- life试剂
- Nunc耗材
- 北京杰辉博高生物技术有限公司
- 联系人:杨经理
- 电话:4000610918
- 邮箱:1261299569@qq.com
- 地址:北京市海淀区乾通天商务楼
- 品牌:国产
- 货号:2358412
- 价格: ¥10/瓶
- 发布日期: 2021-02-19
- 更新日期: 2023-04-25
品牌 | 国产 |
货号 | 2358412 |
用途范围 | 科研实验 |
英文名称 | DNA Extraction Kit 200600/720203/720202 |
纯度 | % |
保质期 | 一年 |
是否进口 | 否 |
The RecoverEase DNA isolation kit quickly isolates high-molecular-weight genomic DNA from a variety of tissues without organic solvent extractions or ethanol precipitation (Figure 1). The procedure involves physical disaggregation of the tissue, coarse filtration, and isolation of the cell nuclei via short centrifugation, followed by incubation with a highly active protease solution containing proteinase K to predigest the cellular proteins. Digestion continues as the DNA preparation is transferred to a convenient, free-floating dialysis cup that allows overnight dialysis of the digested peptides across a semipermeable membrane. After dialysis, the fully hydrated, purified high-molecular-weight DNA is simply collected from the dialysis cup. The resulting DNA, which consistently measures from 100 to 500 kb in length, is ready for immediate use in experimental applications including Southern blot analysis, the polymerase chain reaction (PCR), analysis by pulsed-field electrophoresis, or the recovery of lambda shuttle vectors from transgenic animals.
Figure 1: Recoverease Method vs. Phenol-Chloroform Method of DNA Isolation
Shows Recoverease method takes takes only 17 hours as compated to 3 days for organic extraction.
The benefits of the RecoverEase DNA isolation kit include the early removal of nucleases in the cell cytosol, elimination of toxic organic solvents, such as phenol and chloroform, and the reduction of shearing forces associated with extractions and excessive handling of the DNA. In addition, the DNA is fully hydrated and ready for immediate use after dialysis.
Certain tissues, like bladder and skin, are not amenable to homogenization or nuclei isolation and are thus not recommended starting materials for genomic DNA isolation using the RecoverEase DNA isolation kit.
For Research Use Only. Not for use in diagnostic procedures