Background:

The THUMP (after thiouridine synthases, RNA methylases and pseudouridine synthases) domain is an ancient 100-110 amino acid motif that is found in proteins that are involved in RNA-modification pathways. THUMP domains contain RNA-binding sequences and are thought to deliver RNA modification enzymes to their target substrates. THUMPD1, THUMPD2 and THUMD3 (THUMP domain-containing protein 1, 2 and 3, respectively) are members of the methyltransferase superfamily and each contain one THUMP domain. Both THUMPD2 and THUMPD3 are expressed in tissues throughout the body with highest expression levels in skeletal muscle, spleen, thymus, liver and kidney. Due to the presence of a THUMP domain, the THUMPD proteins are thought to participate in RNA processing events throughout the cell.

Applications:

WB, IHC, IF

Name of antibody:

THUMPD1

Immunogen:

Fusion protein of human THUMPD1

Full name:

THUMP domain containing 1 (THUMPD1), transcript variant 1

Synonyms:

DKFZp686C1054

SwissProt:

Q9NXG2

IHC positive control:

carcinoma of human kidney tissue and human pancreas tissue; carcinoma of human pancreas tissue and adenocarcinoma of human endometrium tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

39 kDa

WB Positive control:

HepG2, Hela, A549, COS7, Jurkat, MCF-7 cell lysates

WB Recommended dilution:

500-2000