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Invitrogen™ Zenon pHrodo iFL IgG Labeling Reagents
  • 品牌:Invitrogen
  • 货号:Z25611
  • 价格: ¥10/盒
  • 发布日期: 2021-06-04
  • 更新日期: 2023-04-25
产品详请
品牌 Invitrogen
货号 Z25611
用途范围 科研实验
纯度 %
保质期 一年
是否进口
Zenon™ pHrodo™ iFL Green-labeled or Red-labeled Fab fragments (i.e. labeling reagent) are directed against the Fc portion of an intact IgG primary antibody to form a labeling complex. Formation of the Fab–antibody complex occurs in fewer than 5 minutes. Because the pHrodo™ iFL dyes dramatically increase in fluorescence as the pH of their surroundings becomes more acidic, the antibodies coupled with the Zenon™ pHrodo™ iFL IgG Labeling Reagents provide an excellent measure of endocytic activity based on the acidification of the labeled antibodies as they are ingested by the cell. 

The Zenon™ pHrodo™ iFL IgG Labeling Reagents contain sufficient material to label one to four 96-well plates, depending on the Zenon™ labeling reagent concentration used. Only a small amount of starting material is needed, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. 

Other advantages of the pH-sensitive Zenon™ pHrodo™ iFL IgG Labeling Reagents include: 

? Simplicity &mdash No reagent purification is required
? Accuracy &mdash No wash steps or quenchers are needed, minimizing cross-reactivity or background
? Speed &mdash Labeled antibodies are typically ready to use in 10 minutes
? Specificity &mdash Dyes are designed for the specific detection of phagocytosis and endocytosis