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Applied Biosystems™ TaqMan? Protein Expression Core Reagents
- 品牌:国产
- 货号:4405501
- 价格: ¥10/盒
- 发布日期: 2021-05-26
- 更新日期: 2023-04-25
产品详请
品牌 | 国产 |
货号 | 4405501 |
用途范围 | 科研实验 |
纯度 | % |
保质期 | 一年 |
是否进口 | 否 |
描述
Applied Biosystems® TaqMan® Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited quantities of cultured hESCs. The TaqMan® Protein Expression Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan® protein expression assays. The Universal PCR Assay contains optimized concentrations of TaqMan® probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems® real-time PCR system. The enzyme concentration, ionic strength, and the pH of the TaqMan® Protein Expression Fast Master Mix are optimized for the amplification of ligated oligonucleotide target sequences prepared using TaqMan® Protein Expression Assay reagents. The DNA polymerase included in the master mix is designed for maximum PCR efficiency and specificity when used with the ligation chemistry. Key features of the TaqMan® Protein Expression Assay:? Quantitates protein expression using gold standard TaqMan® 5’ nuclease chemistry
? Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform
? Allows greater sensitivity and a more quantitative readout than western blots and immunostaining.
For Research Use Only. Not for use in diagnostic procedures.
规格
产品线: | TaqMan™ |
---|---|
技术: | Proximity Ligation Assay |
热启动: | No |
参比荧光染料: | ROX (Pre-mixed) |
聚合酶: | AmpliTaq™ Fast DNA Polymerase |
引物探针兼容性: | TaqMan™ |
PCR 方法: | qPCR Protein Quantification |
纯度或质量等级: | UP (Ultra Pure) |
反应速度: | Fast |
样品类型: | Cells |
靶标特异性: | Not Target-Specific |
检测方法: | Primer-Probe Detection |
适用于(设备): | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
标签或染料: | FAM |
内容及储存
Includes the TaqMan™ Protein Expression Core Reagents Base Kit (with DNA Ligase, Ligase Dilution Buffer, Ligation Reaction Buffer, PBS pH 7.4, Protease, and Universal PCR Assay; store at -15 to -25°C), and the TaqMan™ Protein Expression Fast Master Mix (store at 2 - 8°C).