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Invitrogen™ CellEvent? Caspase 3/7 Green Ready Flow? Reagent
- 品牌:Invitrogen
- 货号:R37167
- 价格: ¥10/盒
- 发布日期: 2021-05-31
- 更新日期: 2023-04-25
产品详请
品牌 | Invitrogen |
货号 | R37167 |
用途范围 | 科研实验 |
纯度 | % |
保质期 | 一年 |
是否进口 | 是 |
描述
CellEvent™ Caspase-3/7 Green Ready Flow™ Reagent enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The product includes 2 reagents: the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent and SYTOX™ AADvanced™ Dead Cell Stain.? Stable at room temperature
? Convenient, ready-to-use format—no need to dilute, weigh, or pipette
? Exceptionally bright green fluorescence upon binding to nucleic acids
? Rapid staining of apoptotic cells without wash steps
CellEvent Caspase-3/7 Green Ready Flow Reagent is a cell-permeant reagent that detects caspase 3/7 activity using flow cytometry. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.
CellEvent Caspase-3/7 Green Ready Flow Reagent consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorescent signal having absorption/emission maxima of ~502/530 nm. When used together with the SYTOX AADvanced Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.
Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.
View more ?
图表
Jurkat cells treated with CellEvent Caspase 3/7 Green reagent
规格
颜色: | Green |
---|---|
激发/发射: | 504/523 |
产品线: | CellEvent™ Ready Flow |
子细胞定位: | Nucleus |
内容及储存
4 dropper bottles, store (at room temperature)