批发供应Sigma Tes生物缓冲液说明书

Tes 生物缓冲液
Sigma T1375 assay ≥99% （titration）
Synonym:别名
2-[（2-Hydroxy-1,1-bis（hydroxymethyl）ethyl）amino]ethanesulfonic acid
N-[Tris（hydroxymethyl）methyl]-2-aminoethanesulfonic acid
TES free acid
Molecular:FormulaC6H15NO6S  分子式
Molecular:Weight229.25    分子量
CAS Number:7365-44-8 Beilstein Registry Number 1957061
EG/EC Number:2309063
MDL number:MFCD00007532
PubChem Substance ID:24899960
 
STORAGE / STABILITY AS SUPPLIED:
    TES free acid is stable at room temperature for at least three years.

SOLUBILITY / STABILITY OF SOLUTIONS:
    TES free acid is very soluble in water; T1375, routinely tested for solution appearance at 33% （w/v）, gives a clear colorless solution. T6541, SigmaUltra, gives a clear colorless solution at 1 M, with a pH range of 3.5-5.0.3 Solutions should be stable at 2-8EC for at least six months. Sterilization should be done by filtration through 0.2 m filters, since generally autoclaving is not recommended for any sulfonic acid buffers. However, autoclavability was checked on 0.5M solutions at pH 6.7, 7.45 and 8.5, and at 0.05M at pH 7.4. The pH was the same between autoclaved and non-autoclaved samples and solutions remained colorless.

GENERAL REMARKS:
    TES is a structural analog to Trizma buffer, one of the ethanesulfonic acid series of biological buffers developed by Good et al., to meet these criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents,minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range, and easily synthesized. Although the structure above is drawn as a neutral molecule, it probably exists in solution as a zwitterion.
    Because the pKa is 7.4 （physiological pH） this buffer has considerable potential for a variety of biological applications.In many culture media that require metal cations, many buffers cannot be used due to chelation or precipitation （citrateor phosphate, for example.） TES was found to be particularly beneficial in a study of succinate oxidation.
    A buffer using TES free acid can be prepared by titrating the free acid （T1375 or SigmaUltra T6541） with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH. Alternatively, solutions of equimolar TES free acid and TES sodium （T0772） can be mixed to attain a buffer of the desired pH. Titrating a solution of TES sodium with HCl is not recommended since the resulting solution will contain a half-equivalent of NaCl.
    Sigma offers a number of TES buffer products: T1375 （reagent grade）, T6541 （SigmaUltra tested for trace metal impurities）, T6022 （cell culture-tested）, and T4152 （molecular biology-tested）. T1030, a hemisodium TES, dissolves in water to produce a buffer with pH approximately 7.4. Please check current catalog listings. 
 

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